other:
Article Title: YAK577 Attenuates Cardiac Remodeling and Fibrosis in Isoproterenol-Infused Heart Failure Mice by Downregulating MMP12.
Article Snippet: Background and Objectives: Heart failure is a potentially fatal event caused by diverse cardiovascular diseases, leading to high morbidity and mortality.. Histone deacetylase (HDAC) inhibitors positively influence cardiac hypertrophy, fibrosis, hypertension, myocardial infarction, and heart failure, causing some side effects.. We aimed to investigate the effect of the novel HDAC inhibitor YAK577 on the heart failure mouse model and its underlying mechanism.
Article Title: Cx43-mediated hyphal folding counteracts phagosome integrity loss during fungal infection.
Article Snippet: The primary antibodies are as follows: anti-C. albicans (catalogue #GTX40096, Gene tex), anti-LAMP1 (catalogue #1D4B, Developmental Studies Hybridoma Bank), anti-Alix (catalogue #sc-53540 Santa Cruz Biotechnology), anti-Tsg101 (catalogue #AB83 Abcam), anti-Galectin3 (catalogue #sc-32790 Santa Cruz Biotechnology), anti-LC3 (catalogue #PA1-16930 Thermo Fisher Scientific), anti-p62 (catalogue #51145 Cell Signalling), anti-Beclin1 (catalogue #A-00023 Sigma), and anti-Cx43 (catalogue #AB0016–500 SICGEN).
Microscopy:
Immunofluorescence:Article Title: Microautophagy regulated by STK38 and GABARAPs is essential to repair lysosomes and prevent aging.
Article Snippet: .. The following antibodies were used in this study: anti-LAMP1 (mouse, 1/1,000; Santa Cruz Biotechnology, sc-20011), anti-Galectin3 (rat, 1/ 1,000; Santa Cruz Biotechnology, sc-23938), anti-STK38 (mouse, 1/ 1,000; Santa Cruz Biotechnology, sc-100404), anti-NDR1/2 (PhosphoThr444/442) (rabbit, 1/1,000; Signalway Antibody, 12521), antiGAPDH (rabbit, 1/64,000; Cell Signaling Technology, 2118), antiCHMP4B (rabbit, 1/1,000; Proteintech, 13683-1-AP), anti-ALIX (for immunofluorescence, mouse, 1/1,000; BioLegend, 634502), anti-VPS4 (rabbit, 1/500–1,000; Sigma-Aldrich, SAB4200025), anti-LC3B (rabbit, 1/1,000; MBL, PM036), anti-a-tubulin (rabbit, 1/64,000; MBL, PM054), anti-GFP (rabbit, 1/1,000; Cell Signaling Technology, 2555), anti-a-tubulin (mouse, 1/10,000; Abcam, ab11304), anti-EGFR (sheep, 1/1,000; Fitzgerald, 20-ES04), anti-Atg5 (rabbit, 1/1,000; Cell Signaling Technology, 12994), anti-Atg13 (rabbit, 1/1,000; Cell Signaling Technology, 13468), anti-ubiquitin Lys48-specific (rabbit, 1/1,000; Millipore, 05-1307), anti-p62 (rabbit, 1/1,000; MBL, PM045), antiCD63 (mouse, 1/500; BD Biosciences, 556019), anti-AHNAK (rabbit, 1/1,000; Proteintech, 16637-1-AP), anti-FLAG (mouse, 1/1,000; Sigma-Aldrich, F1804), anti-HA (rat, 1/1,000; Sigma), anti-RFP (rabbit, 1/1,000; MBL, PM005), anti-ALIX (for western blotting, rabbit, 1/ 1,000; Sigma-Aldrich, ABC40), anti-TSG101 (mouse, 1/1,000; BD Biosciences, 612696), anti-p21 (rabbit, 1/1,000; Abcam, ab109199), antip16 INK4a (mouse, 1 lg/ml; IBL, 11104), anti-LC3A (rabbit, 1/ 1,000; Cell Signaling Technology, 4599), anti-GABARAP (rabbit, 1/ 1,000; MBL, PM037), anti-GABARAPL1 (rabbit, 1/1,000; Abcam, ab86497), anti-GABARAPL2 (rabbit, 1/1,000; MBL, PM038). .. The secondary antibodies used for western blotting were as follows: horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (1:5,000; Jackson ImmunoResearch, 111-035-003), HRP-conjugated goat antimouse IgG (1:5,000; Jackson ImmunoResearch, 115-035-003), HRP-conjugated rabbit anti-sheep IgG (1:2,000; Jackson Invitrogen, 81-8620), HRP-conjugated goat anti-rat IgG (1:5,000; Jackson ImmunoResearch, 112-035-003.
Article Title: Microautophagy regulated by STK38 and GABARAPs is essential to repair lysosomes and prevent aging
Article Snippet: .. The following antibodies were used in this study: anti‐LAMP1 (mouse, 1/1,000; Santa Cruz Biotechnology, sc‐20011), anti‐Galectin3 (rat, 1/1,000; Santa Cruz Biotechnology, sc‐23938), anti‐STK38 (mouse, 1/1,000; Santa Cruz Biotechnology, sc‐100404), anti‐NDR1/2 (Phospho‐Thr444/442) (rabbit, 1/1,000; Signalway Antibody, 12521), anti‐GAPDH (rabbit, 1/64,000; Cell Signaling Technology, 2118), anti‐CHMP4B (rabbit, 1/1,000; Proteintech, 13683‐1‐AP), anti‐ALIX (for immunofluorescence, mouse, 1/1,000; BioLegend, 634502), anti‐VPS4 (rabbit, 1/500–1,000; Sigma‐Aldrich, SAB4200025), anti‐LC3B (rabbit, 1/1,000; MBL, PM036), anti‐α‐tubulin (rabbit, 1/64,000; MBL, PM054), anti‐GFP (rabbit, 1/1,000; Cell Signaling Technology, 2555), anti‐α‐tubulin (mouse, 1/10,000; Abcam, ab11304), anti‐EGFR (sheep, 1/1,000; Fitzgerald, 20‐ES04), anti‐Atg5 (rabbit, 1/1,000; Cell Signaling Technology, 12994), anti‐Atg13 (rabbit, 1/1,000; Cell Signaling Technology, 13468), anti‐ubiquitin Lys48‐specific (rabbit, 1/1,000; Millipore, 05‐1307), anti‐p62 (rabbit, 1/1,000; MBL, PM045), anti‐CD63 (mouse, 1/500; BD Biosciences, 556019), anti‐AHNAK (rabbit, 1/1,000; Proteintech, 16637‐1‐AP), anti‐FLAG (mouse, 1/1,000; Sigma‐Aldrich, F1804), anti‐HA (rat, 1/1,000; Sigma), anti‐RFP (rabbit, 1/1,000; MBL, PM005), anti‐ALIX (for western blotting, rabbit, 1/1,000; Sigma‐Aldrich, ABC40), anti‐TSG101 (mouse, 1/1,000; BD Biosciences, 612696), anti‐p21 (rabbit, 1/1,000; Abcam, ab109199), anti‐p16 INK4a (mouse, 1 μg/ml; IBL, 11104), anti‐LC3A (rabbit, 1/1,000; Cell Signaling Technology, 4599), anti‐GABARAP (rabbit, 1/1,000; MBL, PM037), anti‐GABARAPL1 (rabbit, 1/1,000; Abcam, ab86497), anti‐GABARAPL2 (rabbit, 1/1,000; MBL, PM038). .. The secondary antibodies used for western blotting were as follows: horseradish peroxidase (HRP)‐conjugated goat anti‐rabbit IgG (1:5,000; Jackson ImmunoResearch, 111‐035‐003), HRP‐conjugated goat anti‐mouse IgG (1:5,000; Jackson ImmunoResearch, 115‐035‐003),HRP‐conjugated rabbit anti‐sheep IgG (1:2,000; Jackson Invitrogen, 81‐8620), HRP‐conjugated goat anti‐rat IgG (1:5,000; Jackson ImmunoResearch, 112‐035‐003.
Western Blot:Article Title: Microautophagy regulated by STK38 and GABARAPs is essential to repair lysosomes and prevent aging.
Article Snippet: .. The following antibodies were used in this study: anti-LAMP1 (mouse, 1/1,000; Santa Cruz Biotechnology, sc-20011), anti-Galectin3 (rat, 1/ 1,000; Santa Cruz Biotechnology, sc-23938), anti-STK38 (mouse, 1/ 1,000; Santa Cruz Biotechnology, sc-100404), anti-NDR1/2 (PhosphoThr444/442) (rabbit, 1/1,000; Signalway Antibody, 12521), antiGAPDH (rabbit, 1/64,000; Cell Signaling Technology, 2118), antiCHMP4B (rabbit, 1/1,000; Proteintech, 13683-1-AP), anti-ALIX (for immunofluorescence, mouse, 1/1,000; BioLegend, 634502), anti-VPS4 (rabbit, 1/500–1,000; Sigma-Aldrich, SAB4200025), anti-LC3B (rabbit, 1/1,000; MBL, PM036), anti-a-tubulin (rabbit, 1/64,000; MBL, PM054), anti-GFP (rabbit, 1/1,000; Cell Signaling Technology, 2555), anti-a-tubulin (mouse, 1/10,000; Abcam, ab11304), anti-EGFR (sheep, 1/1,000; Fitzgerald, 20-ES04), anti-Atg5 (rabbit, 1/1,000; Cell Signaling Technology, 12994), anti-Atg13 (rabbit, 1/1,000; Cell Signaling Technology, 13468), anti-ubiquitin Lys48-specific (rabbit, 1/1,000; Millipore, 05-1307), anti-p62 (rabbit, 1/1,000; MBL, PM045), antiCD63 (mouse, 1/500; BD Biosciences, 556019), anti-AHNAK (rabbit, 1/1,000; Proteintech, 16637-1-AP), anti-FLAG (mouse, 1/1,000; Sigma-Aldrich, F1804), anti-HA (rat, 1/1,000; Sigma), anti-RFP (rabbit, 1/1,000; MBL, PM005), anti-ALIX (for western blotting, rabbit, 1/ 1,000; Sigma-Aldrich, ABC40), anti-TSG101 (mouse, 1/1,000; BD Biosciences, 612696), anti-p21 (rabbit, 1/1,000; Abcam, ab109199), antip16 INK4a (mouse, 1 lg/ml; IBL, 11104), anti-LC3A (rabbit, 1/ 1,000; Cell Signaling Technology, 4599), anti-GABARAP (rabbit, 1/ 1,000; MBL, PM037), anti-GABARAPL1 (rabbit, 1/1,000; Abcam, ab86497), anti-GABARAPL2 (rabbit, 1/1,000; MBL, PM038). .. The secondary antibodies used for western blotting were as follows: horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (1:5,000; Jackson ImmunoResearch, 111-035-003), HRP-conjugated goat antimouse IgG (1:5,000; Jackson ImmunoResearch, 115-035-003), HRP-conjugated rabbit anti-sheep IgG (1:2,000; Jackson Invitrogen, 81-8620), HRP-conjugated goat anti-rat IgG (1:5,000; Jackson ImmunoResearch, 112-035-003.
Article Title: Microautophagy regulated by STK38 and GABARAPs is essential to repair lysosomes and prevent aging
Article Snippet: .. The following antibodies were used in this study: anti‐LAMP1 (mouse, 1/1,000; Santa Cruz Biotechnology, sc‐20011), anti‐Galectin3 (rat, 1/1,000; Santa Cruz Biotechnology, sc‐23938), anti‐STK38 (mouse, 1/1,000; Santa Cruz Biotechnology, sc‐100404), anti‐NDR1/2 (Phospho‐Thr444/442) (rabbit, 1/1,000; Signalway Antibody, 12521), anti‐GAPDH (rabbit, 1/64,000; Cell Signaling Technology, 2118), anti‐CHMP4B (rabbit, 1/1,000; Proteintech, 13683‐1‐AP), anti‐ALIX (for immunofluorescence, mouse, 1/1,000; BioLegend, 634502), anti‐VPS4 (rabbit, 1/500–1,000; Sigma‐Aldrich, SAB4200025), anti‐LC3B (rabbit, 1/1,000; MBL, PM036), anti‐α‐tubulin (rabbit, 1/64,000; MBL, PM054), anti‐GFP (rabbit, 1/1,000; Cell Signaling Technology, 2555), anti‐α‐tubulin (mouse, 1/10,000; Abcam, ab11304), anti‐EGFR (sheep, 1/1,000; Fitzgerald, 20‐ES04), anti‐Atg5 (rabbit, 1/1,000; Cell Signaling Technology, 12994), anti‐Atg13 (rabbit, 1/1,000; Cell Signaling Technology, 13468), anti‐ubiquitin Lys48‐specific (rabbit, 1/1,000; Millipore, 05‐1307), anti‐p62 (rabbit, 1/1,000; MBL, PM045), anti‐CD63 (mouse, 1/500; BD Biosciences, 556019), anti‐AHNAK (rabbit, 1/1,000; Proteintech, 16637‐1‐AP), anti‐FLAG (mouse, 1/1,000; Sigma‐Aldrich, F1804), anti‐HA (rat, 1/1,000; Sigma), anti‐RFP (rabbit, 1/1,000; MBL, PM005), anti‐ALIX (for western blotting, rabbit, 1/1,000; Sigma‐Aldrich, ABC40), anti‐TSG101 (mouse, 1/1,000; BD Biosciences, 612696), anti‐p21 (rabbit, 1/1,000; Abcam, ab109199), anti‐p16 INK4a (mouse, 1 μg/ml; IBL, 11104), anti‐LC3A (rabbit, 1/1,000; Cell Signaling Technology, 4599), anti‐GABARAP (rabbit, 1/1,000; MBL, PM037), anti‐GABARAPL1 (rabbit, 1/1,000; Abcam, ab86497), anti‐GABARAPL2 (rabbit, 1/1,000; MBL, PM038). .. The secondary antibodies used for western blotting were as follows: horseradish peroxidase (HRP)‐conjugated goat anti‐rabbit IgG (1:5,000; Jackson ImmunoResearch, 111‐035‐003), HRP‐conjugated goat anti‐mouse IgG (1:5,000; Jackson ImmunoResearch, 115‐035‐003),HRP‐conjugated rabbit anti‐sheep IgG (1:2,000; Jackson Invitrogen, 81‐8620), HRP‐conjugated goat anti‐rat IgG (1:5,000; Jackson ImmunoResearch, 112‐035‐003.
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